Gene Synthesis Services

Why Choose Bon Opus Biosciences for Your Gene Synthesis and Molecular Biology Needs?

Tailored Gene Constructs: Our tailored gene synthesis services allow you to create custom gene sequences. We match your research requirements exactly, ensuring the highest fidelity for your experiments.
Advanced Molecular Techniques: From gene assembly to plasmid DNA preparation, our comprehensive workflow includes cutting-edge molecular biology techniques for every step of your project.
Accuracy and Efficiency: Our rigorous QC processes, error-free cloning, and sequence verification give you confidence in your research outcomes.
Rapid Turnaround Times: Accelerate your research with our quick turnaround, delivering custom gene sequences efficiently to keep pace with your project timelines.
Customizable Solutions: Our molecular biology experts collaborate with you to understand your project's unique needs, providing a truly bespoke service experience.
Comprehensive Support: From vector design to post-synthesis processing, our team offers end-to-end support, including expert consultation and after-sales service.

Sequence optimization

The provided DNA sequence is analyzed by evaluating the GC content and repetitive sequences, and optimizing the codon sequence according to the protein expression system required. (Completed with the Quote)

Oligo Synthesis

To design the oligos required for gene synthesis, oligo analysis software is used to determine how to divide the gene into shorter fragments and design the oligos. The length of the oligos is usually around 100bp. Subsequently, all the oligos are synthesized in one oligonucleotide synthesizer, which is completed by the end of Night 1.

Gene Assembly

The synthesized oligos are assembled into longer fragments, typically 6-700bp, through PCR and PCR assembly. For genes less than 1.5kb, such as mAb seq, the complete gene can be obtained at this step. The fragments are then cloned into PUC57 and sequenced through fragment sequencing to verify their accuracy. For genes more than 1.5kb, a few more rounds of PCR will be used to amplify the entire gene.

Cloning

The synthesized gene is cloned into a plasmid vector by either enzyme digestion or seamless cloning (Gibson).

Sanger sequencing is performed to ensure the correct sequence of the synthesized gene and enzyme digestion to verify the size of resulting DNA fragments. We guarantee 100% accuracy of the synthetic DNA sequences.

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New Year
Better Prices

Gene synthesis starts at $0.19 per bp

1mg maxi prep starts at $99

Site-directed mutagenesis starts at $79

Citations of our works

ApexImmune Therapeutics

Bon Opus has provided us with excellent gene synthesis and protein expression and purification services. Their design team is always accommodating to our requests and highly detailed in their project planning. Their deliverables are also spot on. We look to Bon Opus for all our gene synthesis and protein reagent production needs.

Dr. Qian Zhang,
Scripps Research Institute

The Bon Opus team is truly efficient, experienced and reliable for gene synthesis and cloning. They have successfully helped us synthesize the VH and VL genes from a monoclonal antibody and then clone them into their antibody expression vectors. Their scientists were very attentive and responsive. They even noticed one of the aa sequences I submitted was missing two residues in the very N-terminus so that we could fix the mistake in time. The final products were delivered on time, of great quality, and at very competitive pricing. Overall, we are very satisfied with their service and will continue using their gene synthesis products.

Dr. Ying Wang,
Sorrento Therapeutics

[Bon Opus] provided us with high-quality gene fragment services, even when the sequence had high GC content or a strong secondary structure. There were no mutations involved during the sequencing of our cloned gene in the plasmids. The QC data for their gene synthesis services is exceptionally clear and helpful. I have not yet worked with another company whose data proved to be so valuable. [Bon Opus]’ service is very good overall and I would highly recommend to anyone.